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rnascope ® fluorescent in situ hybridization (fish)  (Advanced Cell Diagnostics Inc)

 
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    Advanced Cell Diagnostics Inc rnascope ® fluorescent in situ hybridization (fish)
    Rnascope ® Fluorescent In Situ Hybridization (Fish), supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnascope ® fluorescent in situ hybridization (fish)/product/Advanced Cell Diagnostics Inc
    Average 90 stars, based on 1 article reviews
    rnascope ® fluorescent in situ hybridization (fish) - by Bioz Stars, 2026-03
    90/100 stars

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    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ <t>hybridization/RNAscope.</t> DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
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    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ <t>hybridization/RNAscope.</t> DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
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    rnascope fluorescence in situ hybridization (fish)  (Jackson Laboratory)
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    Jackson Laboratory rnascope fluorescence in situ hybridization (fish)
    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ <t>hybridization/RNAscope.</t> DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
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    Image Search Results


    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide

    doi: 10.1152/ajpregu.00297.2019

    Figure Lengend Snippet: Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.

    Article Snippet: Coronal sections of 10 μm were cut using a cryostat, and sections underwent RNAscope Multiplex fluorescence in situ hybridization (FISH) for fresh frozen samples (Advanced Cell Diagnostics).

    Techniques: In Situ Hybridization, RNAscope, Staining

    Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide

    doi: 10.1152/ajpregu.00297.2019

    Figure Lengend Snippet: Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.

    Article Snippet: Coronal sections of 10 μm were cut using a cryostat, and sections underwent RNAscope Multiplex fluorescence in situ hybridization (FISH) for fresh frozen samples (Advanced Cell Diagnostics).

    Techniques: In Situ Hybridization, RNAscope